Monday, February 17, 2020

Synthesis of -carbolines as novel CDK4 inhibitors Lab Report

Synthesis of -carbolines as novel CDK4 inhibitors - Lab Report Example Considering the importance of Cyclin-dependent kinases (CDKs), a set of protein kinases, it is essential to explore its mechanism that makes it relevant for the lab process. The kinases aid in regulating the cell cycles, transcription Mrna processing and differentiation of nerve cells. In the function of these kinases, cyclin is crucial considering that it acts as the regulatory protein which binds the CDKs. Consequently, this means that in the absence of cyclin, less kinase activity takes place because of the absence of cyclin-CDK complexes1. CDK4, acting as a member of the cyclin-dependent kinase family, functions as an intrinsic catalyutic protein kinase complex for cell cycle G1 phase progression. D type cyclins and CDK inhibitor are important for the functioning of the kinase since they suppress the action of tumour in causing cell proliferation. The kinase works in phosphorylation of the retinoblastoma gene product thereby helping in preventing the occurring mutation of the genes responsible for the tumuourigenesis of various types of cancer 2. Further, small compouds such as pentacyclic quaternary salt, act as promising factors in the direction for treatment of cancer. The action of the kinase in causing DNA-interchelation can be reduced by ÃŽ ²-carboline which usually acts as a non-planner maintaining the activity of CDK4. Fascaplysin acts as a pentacyclic quaternary salt used as an anti-cancer agent considering its action in suppressing certain leukemia cells in mice. Further, the salt also end up hindering CDK4 leading to arrest of cell cycle in both normal and tumours ncells in the G phase. The hindering process occurs because it binds to theATP pocket of the kinase, resulting to G arrest via a bidentate hydrogen bond donor/acceptor pair. The chain involving phosphorylation of pRb enables the cell to pass through the G1 checkpoint leading to completion of division cycle because of the associated release of E2F proteins.

Monday, February 3, 2020

Fishbowl Inventory Control Solution Research Paper - 1

Fishbowl Inventory Control Solution - Research Paper Example Additionally, it requires setting up the users, customizing the reports, and integrating the QuickBooks with the other applications used by the third parties to align the industry with its particular needs. During the testing process, there will be a utilization of use cases to ensure that there exists maximal functionality of the system. Such cases include the Log on and the Shut down among others. The purpose of the cases is to facilitate communication among the users, understand various requirements, and provide the prototype test cases. User involvement is vital to the testing and implementation of the technology into the company. For maximum participation among the users, it is paramount to involve them as early as the development of the cases starts. The situation will enhance their understanding and minimize time wastage when dealing with the clients. In this case, the immediate program manager will sign off to ascertain the testing of this novel technology. The company needs a development and a production environment since it is an emerging business. However, with time, there is a need to involve such an environment. In this context, the company employees are few, meaning that all of them require training to maximize the available resources. Further, training all the employees will ensure that they acquire the much-needed skills to help one another in the delivery of the company services. However, there will be an emphasis on those that interact with the technology on a daily basis. The training will commence mid the next week on Wednesday the 24 2015 at the company’s boardroom. During the training, special applications will be of help to guarantee an adequate training the acquisition of the technical skills that require an emphasis (Sun-Mee & Munoz, 2014). For instance, the distinct applications intended for use include; customized videos and consultant training sessions.